The identification of DNA methylation by next-generation sequencing (NGS) provides important insights into changes in gene expression. In preparation for NGS, scientists often treat methylated DNA samples with sodium bisulfite, which causes damage and leads to skewed sequence coverage. A new enzymatic conversion method in conjunction with target enrichment by hybrid capture offers a sensitive alternative approach that prepares DNA for methylome analysis in a variety of settings.

Download this application note from Twist Bioscience to learn more about a new workflow for targeted methylation detection.